Open AccessSelective Expansion of Skeletal Muscle Stem Cells from Bulk Muscle Cells in Soft Three‐Dimensional Fibrin Gel
Author(s) -
Zhu Pei,
Zhou Yalu,
Wu Furen,
Hong Yuanfan,
Wang Xin,
Shekhawat Gajendra,
Mosenson Jeffrey,
Wu WenShu
Publication year - 2017
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.1002/sctm.16-0427
Subject(s) - ex vivo , stem cell , skeletal muscle , regenerative medicine , in vivo , cell sorting , microbiology and biotechnology , fibrin , muscle disorder , cell therapy , myocyte , cell , biology , biomedical engineering , immunology , anatomy , medicine , biochemistry
Abstract Muscle stem cells (MuSCs) exhibit robust myogenic potential in vivo, thus providing a promising curative treatment for muscle disorders. Ex vivo expansion of adult MuSCs is highly desired to achieve a therapeutic cell dose because of their scarcity in limited muscle biopsies. Sorting of pure MuSCs is generally required for all the current culture systems. Here we developed a soft three‐dimensional (3D) salmon fibrin gel culture system that can selectively expand mouse MuSCs from bulk skeletal muscle preparations without cell sorting and faithfully maintain their regenerative capacity in culture. Our study established a novel platform for convenient ex vivo expansion of MuSCs, thus greatly advancing stem cell‐based therapies for various muscle disorders. S tem C ells T ranslational M edicine 2017;6:1412–1423