Open AccessIn Situ Tissue Regeneration of Renal Tissue Induced by Collagen Hydrogel Injection
Author(s) -
Lee Sang Jin,
Wang HungJen,
Kim TaeHyoung,
Choi Jin San,
Kulkarni Gauri,
Jackson John D.,
Atala Anthony,
Yoo James J.
Publication year - 2018
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.1002/sctm.16-0361
Subject(s) - renal stem cell , pathology , regeneration (biology) , progenitor cell , synaptopodin , mesenchymal stem cell , kidney , stem cell , cytokeratin , medicine , chemistry , biology , immunohistochemistry , podocyte , microbiology and biotechnology , proteinuria
Abstract Host stem/progenitor cells can be mobilized and recruited to a target location using biomaterials, and these cells may be used for in situ tissue regeneration. The objective of this study was to investigate whether host biologic resources could be used to regenerate renal tissue in situ. Collagen hydrogel was injected into the kidneys of normal mice, and rat kidneys that had sustained ischemia/reperfusion injury. After injection, the kidneys of both animal models were examined up to 4 weeks for host tissue response. The infiltrating host cells present within the injection regions expressed renal stem/progenitor cell markers, PAX‐2, CD24, and CD133, as well as mesenchymal stem cell marker, CD44. The regenerated renal structures were identified by immunohistochemistry for renal cell specific markers, including synaptopodin and CD31 for glomeruli and cytokeratin and neprilysin for tubules. Quantitatively, the number of glomeruli found in the injected regions was significantly higher when compared to normal regions of renal cortex. This phenomenon occurred in normal and ischemic injured kidneys. Furthermore, the renal function after ischemia/reperfusion injury was recovered after collagen hydrogel injection. These results demonstrate that introduction of biomaterials into the kidney is able to facilitate the regeneration of glomerular and tubular structures in normal and injured kidneys. Such an approach has the potential to become a simple and effective treatment for patients with renal failure. S tem C ells T ranslational M edicine 2018;7:241–250