Open AccessCooperativity and intermediate structures of single‐stranded DNA binding‐assisted recA‐single‐stranded DNA complex formation studied by atomic force microscopy
Author(s) -
Umemura K.,
Okada T.,
Kuroda R.
Publication year - 2006
Publication title -
scanning
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.359
H-Index - 47
eISSN - 1932-8745
pISSN - 0161-0457
DOI - 10.1002/sca.4950270107
Subject(s) - dna , cooperativity , atomic force microscopy , chemistry , crystallography , cooperative binding , biophysics , binding site , nanotechnology , biology , materials science , biochemistry
The formation of a complex between RecA protein and single‐stranded (ss) DNA was studied systematically by atomic force microscopy (AFM) by varying incubation time and the molecular ratio of RecA protein to single‐stranded DNA binding (SSB) protein. New intermediate structures, such as small circular, tangled, and protruded structures in the absence of SSB and sharply turned structures in the presence of SSB, were clearly identified at the early stage of complex formation. These structures have probably resulted from competitive binding of RecA and SSB to DNA. After long incubation, only fully covered RecA‐ssDNA and totally RecA‐free SSBssDNA complexes were present regardless of RecA concentrations. Together with intermediate structures which consisted of only two parts, that is, ssDNA covered by SSB and by RecA proteins, the observation suggested strong neighbor cooperative binding of RecA to ssDNA assisted by SSB.