Open AccessThree‐dimensional reconstruction and motion analysis of living, crawling cells
Author(s) -
Soll David R.,
Voss Edward,
Johnson Olof,
Wessels Deborah
Publication year - 2000
Publication title -
scanning
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.359
H-Index - 47
eISSN - 1932-8745
pISSN - 0161-0457
DOI - 10.1002/sca.4950220404
Subject(s) - pseudopodia , crawling , vesicle , cytoplasm , nucleus , process (computing) , biological system , biophysics , cell , motion (physics) , live cell imaging , cell membrane , nanotechnology , computer science , microbiology and biotechnology , biology , materials science , membrane , artificial intelligence , anatomy , actin , operating system , genetics
Abstract Cell behavior is three‐dimensional (3‐D), even when it takes place on a flat surface. Migrating cells form pseudopods on and off the substratum, and the cell body undergoes height changes associated with a 1 min behavior cycle. Inside the cell, the nucleus has a 3‐D migratory cycle, and vesicles move up and down in the z‐axis as a cell locomotes. For these reasons, the two‐dimensional (2‐D) analysis of cellular and subcellular behavior is, in many cases, inadequate. We have, therefore, developed 3‐D motion analysis systems that reconstruct the cell surface, nucleus, pseudopods, and vesicles of living, crawling cells in 3‐D at time intervals as short as 1 s, and compute more than 100 parameters of motility and dynamics morphology at 1‐s intervals. We are now in the process of developing a multimode reconstruction system that will allow us to reconstruct and analyze fluorescently tagged molecular complexes within the differential interference contrast‐imaged subcellular architecture of a crawling cell. These evolving technologies should find wide application for a host of biomedical problems.