Open AccessThermal effects of the electron beam and implications of surface damage in the analysis of bone tissue
Author(s) -
Holmes Jennifer L.,
Bachus Kent N.,
Bloebaum Roy D.
Publication year - 2000
Publication title -
scanning
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.359
H-Index - 47
eISSN - 1932-8745
pISSN - 0161-0457
DOI - 10.1002/sca.4950220403
Subject(s) - thermocouple , cathode ray , materials science , scanning electron microscope , beam (structure) , electron , thermal , electron microscope , bone tissue , biomedical engineering , acceleration voltage , temperature measurement , voltage , electron temperature , composite material , optics , thermodynamics , physics , medicine , quantum mechanics
Electron beam interactions with specimens in the scanning electron microscope (SEM) can lead to increased surface temperatures and damage. These changes may have significant consequences in the analysis of bone tissue. An investigation was performed to measure the surface temperature changes associated with the electron beam on a thermocouple with systematic variations in operating conditions. Probe currents, magnifications, and accelerating voltages were incrementally adjusted to measure the temperature changes and to make assessments for determining optimal operating conditions for the SEM in future analyses of bone tissue. Results from this study suggest that thermal effects were minimal at lower accelerating voltages (< 20 kV), lower probe currents (< 10 nA), and lower magnifications, but surface damage may still occur during the analysis of bone tissue.