Open AccessLengths measurements in microvascular corrosion castings: Two‐dimensional versus three‐dimensional morphometry
Author(s) -
Minnich Bernd,
Lametschwandtner Alois
Publication year - 2000
Publication title -
scanning
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.359
H-Index - 47
eISSN - 1932-8745
pISSN - 0161-0457
DOI - 10.1002/sca.4950220305
Subject(s) - scanning electron microscope , morphology (biology) , anatomy , materials science , morphometrics , orientation (vector space) , biomedical engineering , corrosion , biology , geometry , composite material , mathematics , medicine , genetics , fishery
In the present study we compared measurements of vessel lengths from (a) single‐digital scanning electron microscope (SEM) images of microvascular corrosion casts (VCCs) of gill filters of tadpoles of Xenopus laevis Daudin by two‐dimensional (2‐D) morphometry (Optimas 6.5, Optimas Corp., Bothell, Wash., USA; planar measurements) and (b) digital stereopairs by three‐dimensional (3‐D) morphometry (3D‐Morphometry, Minnich and Muska OEG, Salzburg). Depending on the spatial orientation of the vessels measured, we found a maximum difference of 58.84% (100 [3‐D]‐41.16 [2‐D]) in vessel lengths by 3‐D morphometry versus 2‐D morphometry, which, in multiple (segmental) lengths measurements or when determining space angles, might be even higher. Based on results we consider 3‐D morphometry of VCCs to be the method of choice for lengths measurements.