Open AccessNanodissection and noncontact imaging of plasmid DNA with an atomic force microscope
Author(s) -
Geisler Bernd,
Noll Frank,
Hampp Norbert
Publication year - 2000
Publication title -
scanning
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.359
H-Index - 47
eISSN - 1932-8745
pISSN - 0161-0457
DOI - 10.1002/sca.4950220102
Subject(s) - mica , atomic force microscopy , dna , materials science , plasmid , microscopy , microscope , conductive atomic force microscopy , scanning force microscopy , nanotechnology , optics , chemistry , composite material , physics , biochemistry
In this paper we report that a combination of noncontact and contact atomic force microscopy is a convenient and reliable method for imaging and dissecting single plasmid deoxyribonucleic acid (DNA) strands on mica at ambient conditions without leaving feedback and without damage to the scanning tips. The width and thickness measured at different points of the DNA strands agree with literature data and are the same before and after dissection.