Open AccessCorrelative light and backscattered electron microscopy of bone—Part I: Specimen preparation methods
Author(s) -
Goldman Haviva M.,
Kindsvater John,
Bromage Timothy G.
Publication year - 1999
Publication title -
scanning
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.359
H-Index - 47
eISSN - 1932-8745
pISSN - 0161-0457
DOI - 10.1002/sca.4950210106
Subject(s) - scanning electron microscope , microscopy , materials science , electron microscope , correlative , optical microscope , adhesive , optics , biomedical engineering , composite material , medicine , linguistics , philosophy , physics , layer (electronics)
A method for preparing nondecalcified bone and tooth specimens for imaging by both light microscopy (LM) and backscattered electron microscopy in the scanning electron microscope (B SE‐SEM) is presented. Bone blocks are embedded in a polymethylmethacrylate (PMMA) mixture and mounted on glass slides using components of a light‐cured dental adhesive system. This method of slide preparation allows correlative studies to be carried out between different microscopy modes, using the same histologic section. It also represents a large time savings relative to other mounting methods whose media require long cure times.