Open AccessA multiple technical approach to the study of apoptotic cell micronuclei
Author(s) -
Columbaro M.,
Gobbi P.,
Renò F.,
Luchetti F.,
Santi S.,
Valmori A.,
Falcieri E.
Publication year - 1998
Publication title -
scanning
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.359
H-Index - 47
eISSN - 1932-8745
pISSN - 0161-0457
DOI - 10.1002/sca.4950200802
Subject(s) - micronucleus test , cytochemistry , chromatin , biophysics , confocal microscopy , apoptosis , confocal , dna , micronucleus , in situ , electron microscope , scanning electron microscope , microscopy , microbiology and biotechnology , chemistry , dna damage , materials science , ultrastructure , biology , optics , anatomy , biochemistry , physics , organic chemistry , composite material , toxicity
Apoptotic micronuclei have been studied, in different cell types, from a morphologic and functional point of view. Conventional electron microscopy, in various staining conditions, selective cytochemistry for DNA, and freeze fracture for the analysis of chromatin fiber organization and size were performed. In situ TdT and Pol I immunofluorescent techniques were carried out to detect double‐ and single‐strand DNA breaking points by confocal laser scanning microscopy. Apoptotic cell ultrathin cryosections were also performed and were analysed by field emission in lens scanning electron microscopy. Double/single strand massively cleaved DNA was detected in micronuclei, with a highly supercoiled, uniformly packed, very dense arrangement.