Open AccessThe attachment of mineralised tissues to coverslips for observing dynamic events by confocal microscopy
Author(s) -
Griffiths Brigitte M.,
Watson T. F.
Publication year - 1994
Publication title -
scanning
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.359
H-Index - 47
eISSN - 1932-8745
pISSN - 0161-0457
DOI - 10.1002/sca.4950160107
Subject(s) - confocal , microscope , oil immersion , materials science , confocal microscopy , microscopy , optical microscope , confocal laser scanning microscopy , adhesive , biomedical engineering , scanning electron microscope , optics , composite material , layer (electronics) , medicine , physics
In confocal scanning optical fluorescence microscopy, using high‐aperture oil immersion lenses, the best images are obtained for focus planes immediately under the coverslip, or under a continuation of the coverslip with a medium with the same, high refractive index. Therefore placing the coverslip with a layer of oil underneath it is an advantage if the sample will allow it. If experimental manipulations that can displace the coverslip are involved, —for example, attempts to make dynamic observation of fluid flow—the coverslip is best cemented to the sample with a high‐index material. This report describes the use of adhesive systems developed for restorative dentistry to achieve a durable attachment of dental tissues to microscope coverslips. The technique described in this paper has been used for monitoring real‐time fluid movement in dentine. The samples were examined with a high‐frame‐rate confocal microscope (a tandem scanning microscope). The adhesive technology also could be utilised in the microscopic preparation of other porous translucent materials.