Open AccessLow‐temperature scanning electron microscopy in fungus‐nematode interaction
Author(s) -
Den Belder E.,
Boekestein A.,
Van Esch J. W. J.,
Thiel F.
Publication year - 1993
Publication title -
scanning
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.359
H-Index - 47
eISSN - 1932-8745
pISSN - 0161-0457
DOI - 10.1002/sca.4950150106
Subject(s) - scanning electron microscope , nematode , fungus , root knot nematode , materials science , environmental scanning electron microscope , electron microscope , botany , chemistry , biology , composite material , optics , ecology , physics
A comparative study has been made of different preparation techniques used for the scanning electron microscope (SEM), with regard to their application to fungus‐nematode interaction. The preparation of frozen‐hydrated specimens of both healthy and Arthrobotrys‐oli‐gospora ‐infected second‐stage larvae of the root‐knot nematode ( Meloidogyne sp.) is described, and the results are compared with those obtained by critical point‐drying and freeze‐drying. In all cases the frozen‐hydrated specimens consistently showed the best preservation.