Open AccessDesign and use of a computer controlled confocal microscope for biological applications
Author(s) -
van der Voort H. T. M.,
Brakenhoff G. J.,
Valkenburg J. A. C.,
Nanninga N.
Publication year - 1985
Publication title -
scanning
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.359
H-Index - 47
eISSN - 1932-8745
pISSN - 0161-0457
DOI - 10.1002/sca.4950070202
Subject(s) - microscope , confocal , optics , confocal laser scanning microscope , computer science , resolution (logic) , materials science , detector , optical sectioning , biological imaging , laser scanning , computer vision , laser , artificial intelligence , fluorescence , physics
In the confocal scanning light microscope where the object is scanned mechanically through a finely focused laser spot, a fundamentally higher resolution can be achieved when a point detector is employed. Such a microscope possesses in addition to a high dynamic range excellent sectioning capabilities, and is because of this very suitable for “3‐Dimensional imaging”, especially when operated in fluorescence. An instrument under computer control and with extensive image processing capabilities is described. Various biological applications are given including computer generated stereo images of biological structures with submicron resolution.