Open AccessReal‐time in vivo imaging collagen in lymphedematous skin using multiphoton microscopy
Author(s) -
Wu Xiufeng,
Zhuo Shuangmu,
Chen Jianxin,
Liu Ningfei
Publication year - 2011
Publication title -
scanning
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.359
H-Index - 47
eISSN - 1932-8745
pISSN - 0161-0457
DOI - 10.1002/sca.20266
Subject(s) - in vivo , lymphedema , two photon excitation microscopy , biomedical engineering , multiphoton fluorescence microscope , pathology , microscopy , medicine , preclinical imaging , chemistry , fluorescence microscope , materials science , fluorescence , biology , optics , physics , microbiology and biotechnology , cancer , breast cancer
Changes of dermal collagen are characteristic for chronic lymphedema. To evaluate these changes, a real‐time imaging based on two‐photon excited fluorescence and second‐harmonic generation was developed for investigating collagen of lymphedematous mouse and rat tail skin in vivo . Our findings showed that the technique could image the morphological changes and distribution of collagen in lymphedematous mouse and rat tail skin in vivo . More importantly, it may allow visualization of dynamic collagen alteration during the progression of lymphedema. Our findings demonstrated that multiphoton microscopy may have potential in a clinical setting as an in vivo diagnostic and monitoring system for therapy in lymphology. SCANNING 33:463–467, 2011. © 2011 Wiley Periodicals, Inc.