Open AccessDip‐pen nanolithography on SiO x and tissue‐derived substrates: comparison with multiple biological inks
Author(s) -
Kramer Marcus A.,
Park Heyjin C.,
Ivanisevic Albena
Publication year - 2009
Publication title -
scanning
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.359
H-Index - 47
eISSN - 1932-8745
pISSN - 0161-0457
DOI - 10.1002/sca.20160
Subject(s) - nanolithography , fibronectin , nanotechnology , biomolecule , materials science , dip pen nanolithography , extracellular matrix , substrate (aquarium) , lithography , tissue engineering , adhesion , nanoscopic scale , chemistry , biomedical engineering , biochemistry , composite material , optoelectronics , fabrication , medicine , alternative medicine , oceanography , pathology , geology
Abstract There has been extensive interest in the micro and nanoscale manipulation of various substrates in the past few decades. One promising technique is dip‐pen nanolithography which has shown the capability to pattern substrates of all forms including, tissue‐derived substrates. Patterning of tissue‐derived substrates is of particular interest, as it would facilitate studies into controlling cell morphology and cell–substrate interaction. To expand the field into this area both peptides and bioactive collagen‐binding peptide‐linked biomolecules were patterned to the inner collagenous zone of the Bruch's membrane (BM). Collagen‐binding peptide, and extra cellular matrix (ECM) proteins laminin and fibronectin were patterned on the BM and SiO x . The lithographic protocol was facilitated by Triton X‐100 which was used to clean the tissue‐derived construct after harvesting. This produced a collagen‐exposed BM which was more hydrophilic (contact angle 67°±8.49°) surface compared with other cleaning methods but it maintained similar surface roughness (root‐mean‐square) 80±18 nm and collagen exposure. This type of surface can be readily patterned with the chosen inks under lower humidity conditions. SCANNING 32: 30–34, 2010. © 2009 Wiley Periodicals, Inc.