Open AccessConfocal laser scanning microscopy of chondrocytes in vitro: Cytoskeletal changes after quinolone treatment
Author(s) -
Walter I.,
Egerbacher M.,
Wolfesberger B.,
Seiberl G.
Publication year - 1998
Publication title -
scanning
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.359
H-Index - 47
eISSN - 1932-8745
pISSN - 0161-0457
DOI - 10.1002/sca.1998.4950200705
Subject(s) - cytoskeleton , vimentin , confocal microscopy , confocal , in vitro , microbiology and biotechnology , chemistry , confocal laser scanning microscopy , chondrocyte , stress fiber , cartilage , immunofluorescence , quinolone , integrin , biophysics , actin , actin cytoskeleton , cell culture , cell , pathology , anatomy , antibiotics , biology , immunology , medicine , antibody , biochemistry , immunohistochemistry , optics , physics , genetics
The use of quinolone antibiotics would be significant for chronically diseased children (e.g., cysticfibrosis) as a prophylactic long‐term treatment. However, quinolones were shown to cause cartilage damage in experimental animals when administered during certain developmental stages. In the present study, the effect of quinolones on chondrocytes was studied in a cell culture model in order to avoid animal experiments, to investigate the influence of single factors, and to open up the possibility to test human tissue. Chondrocytes were obtained from hipjoint cartilage of 3 to 4‐weeks‐old rats and cultured in control medium or quinolone‐supplemented medium. It was shown that quinolones heavily disturbed adhesion of chondrocytes to the culture dish, accompanied by changes in cell shape and cytoskeletal morphology. Reduction of filamentous actin (stress fibers) and disintegration of vimentin fibers was demonstrated by immunofluorescence and evaluated by con‐focal laser scanning microscopy. In contrast, distribution and amount of the adhesion molecule integrin α did not change. Results of the present study indicate that quinolones disturb the adherence mechanism of chondrocytes and lead to cytoskeleton changes.