Open AccessCharacterization of biomolecule immobilization by scanning force microscopy using a wet‐masking technique
Author(s) -
Fritzsche Wolfgang,
Ermantraut Eugen,
Köhler J. Michael
Publication year - 1998
Publication title -
scanning
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.359
H-Index - 47
eISSN - 1932-8745
pISSN - 0161-0457
DOI - 10.1002/sca.1998.4950200208
Subject(s) - masking (illustration) , materials science , biomolecule , monolayer , substrate (aquarium) , streptavidin , adsorption , nanotechnology , microscopy , scanning probe microscopy , surface modification , scanning ion conductance microscopy , scanning electron microscope , chemical engineering , chemistry , composite material , optics , biotin , scanning confocal electron microscopy , art , biochemistry , oceanography , physics , organic chemistry , engineering , geology , visual arts
The assemblage of molecular layers was investigated using scanning force microscopy (SFM). A wet‐masking technique was used for the preparation of monolayer steps by partial masking of the substrate with an elastomeric mask during incubation in the modification buffers. The subsequent adsorption of biotin, streptavidin, and biotiny lated beads onto a gold substrate was investigated by SFM visualization of the created steps. The molecular layers were characterized based on measurements of step height and surface roughness. The simultaneous visualization of the surface before and after modification minimizes artifacts introduced by changes in tip shape or imaging parameters.