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Comparison of planktonic and reed biofilm bacteria in different riverine water bodies of river Danube
Author(s) -
Borsodi Andrea K.,
Anda Dóra,
Krett Gergely,
Megyes Melinda,
Németh Kitti,
Dobosy Péter,
Aszalós Júlia Margit,
Engloner Attila
Publication year - 2020
Publication title -
river research and applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.679
H-Index - 94
eISSN - 1535-1467
pISSN - 1535-1459
DOI - 10.1002/rra.3597
Subject(s) - phragmites , biofilm , plankton , water column , bacteria , biology , environmental science , littoral zone , temperature gradient gel electrophoresis , ecology , botany , wetland , 16s ribosomal rna , genetics
As the submerged shoot parts of common reed ( Phragmites australis ) can provide large surfaces for biofilm‐forming bacteria, extensive reed stands can effectively contribute to the self‐purification in the littoral zones of freshwaters. However, bacterial biofilm developed on reed surfaces have been studied only in still waters. In the present study, comparative examinations were performed on the planktonic and reed biofilm bacterial communities from two different riverine water bodies. Water and reed biofilm samples were taken at the beginning and end of the vegetation period from (a) a regulated side arm and (b) a separated oxbow of River Danube. Besides measuring physical and chemical environmental variables, planktonic bacterial cell numbers were detected by 4′,6‐diamidino‐2‐phenylindole staining method, and the bacterial communities were compared by denaturing gradient gel electrophoresis. Our results demonstrated that the structure and composition of bacterial communities correlated with different environmental variables and were different regarding both the habitats (planktonic vs. reed biofilm) and the types of riverine water bodies. The seasonal differences in the bacterial community structures were smaller in the river side arm than in the separated oxbow. In the water column, representatives of widespread and typical freshwater planktonic bacteria (“ Candidatus Planktophila,” Limnohabitans , and Polynucleobacter ), whereas, in the reed biofilm samples, phototrophic and plant associated bacteria were identified.