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Harnessing CRISPR/Cas 9 System for manipulation of DNA virus genome
Author(s) -
Ebrahimi Saeedeh,
Teimoori Ali,
Khanbabaei Hashem,
Tabasi Maryam
Publication year - 2019
Publication title -
reviews in medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.06
H-Index - 90
eISSN - 1099-1654
pISSN - 1052-9276
DOI - 10.1002/rmv.2009
Subject(s) - crispr , cas9 , genome editing , computational biology , biology , genome , palindrome , genomics , dna , genetics , gene
Summary The recent development of the Clustered Regularly Interspaced Palindromic Repeat (CRISPR)/CRISPR‐associated protein 9 (Cas9) system, a genome editing system, has many potential applications in virology. The possibility of introducing site specific breaks has provided new possibilities to precisely manipulate viral genomics. Here, we provide diagrams to summarize the steps involved in the process. We also systematically review recent applications of the CRISPR/Cas9 system for manipulation of DNA virus genomics and discuss the therapeutic potential of the system to treat viral diseases.