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Adeno‐associated virus (AAV) vectors in gene therapy: immune challenges and strategies to circumvent them
Author(s) -
Hareendran Sangeetha,
Balakrishnan Balaji,
Sen Dwaipayan,
Kumar Sanjay,
Srivastava Alok,
Jayandharan Giridhara R.
Publication year - 2013
Publication title -
reviews in medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.06
H-Index - 90
eISSN - 1099-1654
pISSN - 1052-9276
DOI - 10.1002/rmv.1762
Subject(s) - immune system , immunogenicity , vector (molecular biology) , adeno associated virus , biology , genetic enhancement , virology , viral vector , immunology , capsid , acquired immune system , innate immune system , virus , antibody , gene , genetics , recombinant dna
SUMMARY AAV‐based gene transfer protocols have shown remarkable success when directed to immune‐privileged sites such as for retinal disorders like Lebers congenital amaurosis. In contrast, AAV‐mediated gene transfer into liver or muscle tissue for diseases such as hemophilia B, α1 anti‐trypsin deficiency and muscular dystrophy has demonstrated a decline in gene transfer efficacy over time. It is now known that in humans, AAV triggers specific pathways that recruit immune sensors. These factors initiate an immediate reaction against either the viral capsid or the vector encoded protein as part of innate immune response or to produce a more specific adaptive response that generates immunological memory. The vector‐transduced cells are then rapidly destroyed due to this immune activation. However, unlike other viral vectors, AAV is not immunogenic in murine models. Its immunogenicity becomes apparent only in large animal models and human subjects. Moreover, humans are natural hosts to AAV and exhibit a high seroprevalence against AAV vectors. This limits the widespread application of AAV vectors into patients with pre‐existing neutralising antibodies or memory T cells. To address these issues, various strategies are being tested. Alternate serotype vectors (AAV1‐10), efficient expression cassettes, specific tissue targeting, immune‐suppression and engineered capsid variants are some approaches proposed to minimise this immune stimulation. In this review, we have summarised the nature of the immune response documented against AAV in various pre‐clinical and clinical settings and have further discussed the strategies to evade them. Copyright © 2013 John Wiley & Sons, Ltd.