Open AccessEffect of docosahexaenoic acid on in vitro growth of bovine oocytes
Author(s) -
Nagata Shuta,
Tatematsu Kaoru,
Yamaguchi Hitoki,
Inoue Yuki,
Tanaka Keisuke,
Tasaki Hidetaka,
Shirasuna Koumei,
Iwata Hisataka
Publication year - 2021
Publication title -
reproductive medicine and biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.005
H-Index - 22
eISSN - 1447-0578
pISSN - 1445-5781
DOI - 10.1002/rmb2.12403
Subject(s) - docosahexaenoic acid , oocyte , antral follicle , andrology , chemistry , reactive oxygen species , in vitro maturation , in vitro , apoptosis , biology , ovary , fatty acid , biochemistry , endocrinology , microbiology and biotechnology , polyunsaturated fatty acid , embryo , medicine
Purpose The present study investigated the effects of docosahexaenoic acid (DHA) on the growth of bovine oocytes. Methods Oocytes and granulosa cell complexes (OGCs) were collected from early antral follicles (0.4‐0.7 mm) on the surface of ovaries harvested from a slaughterhouse. The OGCs were cultured with 0, 1, and 10 μmol/L docosahexanoic acid (DHA) for 16 days. Results Antrum formation of the OGCs and the number of granulosa cells (GCs) surrounding the oocytes were comparable among groups, whereas supplementation of 0.1 μmol/L of DHA significantly improved oocyte growth. Oocytes grown with DHA had a higher fertilization rate, acetylation levels of H4K12, and ATP contents, as well as a lower lipid content compared with those grown without DHA. In addition, GCs surrounding OGCs grown with DHA had low lipid content compared with vehicle counterparts. Furthermore, when GCs were cultured in vitro, DHA increased ATP production, mitochondrial membrane potential, and reduced lipid content and levels of reactive oxygen species. RNA‐seq of GCs revealed that DHA increased CPT1A expression levels and affect genes associated with focal adhesion, oxidative phosphorylation, and PI3K‐AKT etc Conclusion The results suggest that DHA supplementation affects granulosa cell characteristics and supports oocyte growth in vitro.