Open AccessEffects of estradiol on in vitro maturation of buffalo and goat oocytes
Author(s) -
Maksura Hurum,
Akon Narsisa,
Islam Md Nuronnabi,
Akter Ireen,
Modak Avijit Kumar,
Khatun Asma,
Alam Md Hasanur,
Hashem Md Abul,
Amin Md Ruhul,
Moniruzzaman Mohammad
Publication year - 2021
Publication title -
reproductive medicine and biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.005
H-Index - 22
eISSN - 1447-0578
pISSN - 1445-5781
DOI - 10.1002/rmb2.12350
Subject(s) - in vitro maturation , oocyte , antral follicle , andrology , in vitro , meiosis , oogenesis , biology , chemistry , ovary , endocrinology , embryo , medicine , microbiology and biotechnology , genetics , gene
Purpose The effects of estradiol on oocyte development seem to be varied among species. The present study investigated the effects of 17β‐estradiol on in vitro maturation of buffalo and goat oocytes. Methods Cumulus oocyte complexes (COCs) were aspirated from large antral follicles of slaughtered buffalo and goat ovaries. COCs were cultured in TCM‐199 medium supplemented with 0, 0.5, 1, and 1.5 µg/mL of 17β‐estradiol for in vitro maturation. Then, oocytes were used for the examination of state of nuclear maturation and cumulus expansion. Results In both species, oocytes treated with 17β‐estradiol showed higher cumulus expansion rate than control (0 µg/mL treated). In buffalo, the percentage of oocytes matured to the metaphase II (MII) stage increased in the concentration‐dependent manner of 17β‐estradiol. Similarly, estradiol positively influenced nuclear maturation of goat oocytes in vitro. Conclusions Estradiol has promoting effects on normalprogress of in vitro oocyte meiosis in buffalos and goats.