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An Improved Technique for Determining Abundance of Polycyclic Aromatic Hydrocarbon Degrading Microbial Populations
Author(s) -
Guerin Turlough F.
Publication year - 1999
Publication title -
remediation journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.762
H-Index - 27
eISSN - 1520-6831
pISSN - 1051-5658
DOI - 10.1002/rem.3440100108
Subject(s) - bioremediation , polycyclic aromatic hydrocarbon , environmental remediation , abundance (ecology) , enumeration , environmental science , environmental chemistry , biochemical engineering , groundwater , hydrocarbon , contamination , chemistry , ecology , biology , engineering , mathematics , organic chemistry , geotechnical engineering , combinatorics
The current study describes an improved method for estimating the abundance of polycyclic aramatic hydrocarbon (PAH) degraders in contaminated soil and groundwater. Since the method is a simple incremental improvement to a commonly used approach, it can be easily introduced into the remediation practitioner's testing protocols by simply changing growth indicator dyes. The procedure described is relatively easy to conduct and provides an important addition to laboratories that are using conventional, nonmolecular techniques for microbial enumeration in their bioremediation programs. © 1999 John Wiley & Sons, Inc.