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Destruction of Aspergillus versicolor, Penicillium chrysogenum, Stachybotrys chartarum , and Cladosporium cladosporioides spores using chemical oxidation treatment process
Author(s) -
Yaari Galia,
Tachiev Georgio,
Dean Timothy R.,
Betancourt Doris,
Long Stephanie
Publication year - 2007
Publication title -
remediation journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.762
H-Index - 27
eISSN - 1520-6831
pISSN - 1051-5658
DOI - 10.1002/rem.20127
Subject(s) - penicillium chrysogenum , hydrogen peroxide , cladosporium cladosporioides , spore , chemistry , aspergillus versicolor , nuclear chemistry , microbiology and biotechnology , organic chemistry , aspergillus , botany , biochemistry , biology
The survival of aqueous suspensions of Penicillium chrysogenum, Stachybotrys chartarum, Aspergillus versicolor , and Cladosporium cladosporioides spores was evaluated using various combinations of hydrogen peroxide and Fe 2+ as catalyst. Spore concentrations of 10 6 –10 7 colony forming units per milliliter (CFU/mL) were suspended in water and treated with initial hydrogen peroxide and iron concentrations ranging from 0.05 to 10 percent and 100 to 200 ppm, respectively. After four hours of reaction time, samples were plated on agar plates, and the viable fraction of spores was determined by the number of colonies formed. Hydrogen peroxide concentrations above 50,000 ppm resulted in greater than 6‐log 10 reduction of viable spores for both catalyzed and noncatalyzed reactions. Iron had a strong catalytic effect when added to solutions with hydrogen peroxide concentration above 5,000 ppm and resulted in two to three orders of magnitude greater reduction compared to hydrogen peroxide alone. Additional samples taken after 24 hours of reaction time showed that the effect of the addition of 100 and 200 ppm of Fe 2+ catalyst was mostly kinetic, and noncatalyzed hydrogen peroxide had sporicidal effects similar to catalyzed hydrogen peroxide. This study identified initial reagent concentrations of hydrogen peroxide and Fe 2+ that accomplish a 6‐log 10 reduction of viable mold spores within reaction times of 4 and 24 hours. © 2007 Wiley Periodicals, Inc.