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Primary structural determination of N‐terminally blocked peptides from the bark of Eucommia ulmoides Oliv by mass spectrometric analysis
Author(s) -
Huang RenHuai,
Zhang Ying,
Wang DaCheng
Publication year - 2003
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.999
Subject(s) - chemistry , fourier transform ion cyclotron resonance , mass spectrometry , eucommia ulmoides , chromatography , tandem mass spectrometry , protein mass spectrometry , top down proteomics , edman degradation , sample preparation in mass spectrometry , fast atom bombardment , tandem mass tag , peptide sequence , electrospray ionization , proteomics , biochemistry , quantitative proteomics , medicine , alternative medicine , traditional chinese medicine , pathology , gene
Sequencing of N‐terminally blocked proteins/peptides is a challenge since these molecules inhibit processing by Edman degradation. By using high accuracy Fourier transform ion cyclotron resonance (FTICR) tandem mass spectrometry and matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS), the primary structures of two novel N‐terminally blocked antifungal peptides (EAFP1 and EAFP2), purified from the bark of Eucommia ulmoides Oliv, have been determined. The results show that the high mass accuracy provided by FTICR mass spectrometry is effective to determine the N‐terminally blocking group, and can simplify the task of spectral interpretation and improve the precision of sequence determination. The combination of MALDI‐TOFMS with carboxyl peptidase Y digestion was used to determine the C‐terminal 36‐ and 27‐residue sequences of EAFP1 and EAFP2, respectively, to provide the sequence linkage information for tryptic fragments. Compared with traditional peptide chemistry the advantage of mass spectrometric techniques is their simplicity, speed and sensitivity. Copyright © 2003 John Wiley & Sons, Ltd.