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Some aspects of the stability of ion current in a matrix‐assisted laser desorption/ionization source
Author(s) -
Ptasińska Sylwia,
Michalak Leszek,
Smolira Marcin
Publication year - 2003
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.995
Subject(s) - chemistry , gentisic acid , desorption , matrix (chemical analysis) , benzoic acid , ion , analytical chemistry (journal) , sample preparation , ion source , ionization , chromatography , laser , inorganic chemistry , organic chemistry , salicylic acid , biochemistry , adsorption , physics , optics
The effect of laser exposure (the number of laser shots) on the stability of production of matrix (gentisic acid, sinapinic acid, nicotinic acid and benzoic acid) and biomolecule (insulin and cytochrome‐c) ions is presented. Two methods of sample preparation, dried‐droplet and pressure, were examined. In the case of pressure preparation, the dry powder sample was pressed mechanically with a pressure of 30 MPa. The best results were obtained for the pressure method and gentisic acid as matrix. Copyright © 2003 John Wiley & Sons, Ltd.