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Quantification of acetaldehyde released by lung cancer cells in vitro using selected ion flow tube mass spectrometry
Author(s) -
Smith David,
Wang Tianshu,
SuléSuso Josep,
Španěl Patrik,
Haj Alicia El
Publication year - 2003
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.984
Subject(s) - acetaldehyde , chemistry , mass spectrometry , cell culture , chromatography , in vitro , cell , cancer cell , analytical chemistry (journal) , ethanol , biochemistry , cancer , medicine , biology , genetics
The production of volatile compounds from cancer cell lines in vitro has been investigated using selected ion flow tube mass spectrometry (SIFT‐MS). This technique enables on‐line quantitative analyses of the headspace above cell/medium cultures. This paper reports the discovery that acetaldehyde is released by the lung cancer cell lines SK‐MES and CALU‐1. The concentration of acetaldehyde in the headspace of the medium/cell culture was measured after 16 h incubation at 37°C and found to be proportional to the number of cancer cells in the medium (typically 10 8 ). From these data, the acetaldehyde production rates of the SK‐MES cells and the CALU‐1 cells in vitro are determined to be 1 × 10 6 and 1.5–3 × 10 6 molecules/cell/min, respectively. The potential value of this new technique in cell biology and in industrial cell biotechnology is discussed. Copyright © 2003 John Wiley & Sons, Ltd.