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Atmospheric pressure chemical ionisation reversed‐phase liquid chromatography/ion trap mass spectrometry of intact bacteriohopanepolyols
Author(s) -
Talbot Helen M.,
Squier Angela H.,
Keely Brendan J.,
Farrimond Paul
Publication year - 2003
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.974
Subject(s) - chemistry , mass spectrometry , chromatography , atmospheric pressure chemical ionization , atmospheric pressure , ion trap , liquid chromatography–mass spectrometry , analytical chemistry (journal) , chemical ionization , high performance liquid chromatography , ion , ionization , organic chemistry , oceanography , geology
Abstract Atmospheric pressure chemical ionisation liquid chromatography/multi‐stage ion trap mass spectrometry (APCI‐LC/MS n ) has been applied to the study of intact bacteriohopanepolyols. Spectral characterisation of bacteriohopanepolyols of known structure present in bacterial extracts ( Zymomonas mobilis and a fermenter containing methanotrophs including Methylococcus capsulatus ) has revealed greater structural detail than previous liquid chromatography/mass spectrometry (LC/MS) methods and identified characteristic fragmentations indicative of numerous biohopanoid structures. Analysis of a Recent sedimentary extract from Lake Druzhby (Antarctica) has demonstrated the power of this technique to detect biohopanoids in complex samples including at least partial characterisation of previously unknown composite structures. Copyright © 2003 John Wiley & Sons, Ltd.