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Improved detection of higher molecular weight proteins by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry on polytetrafluoroethylene surfaces
Author(s) -
Botting Catherine H.
Publication year - 2003
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.954
Subject(s) - chemistry , mass spectrometry , desorption , polytetrafluoroethylene , surface enhanced laser desorption/ionization , matrix assisted laser desorption/ionization , matrix (chemical analysis) , matrix assisted laser desorption electrospray ionization , ionization , analytical chemistry (journal) , chromatography , time of flight mass spectrometry , sample preparation in mass spectrometry , laser , protein mass spectrometry , atmospheric pressure laser ionization , electrospray ionization , organic chemistry , ion , photoionization , adsorption , optics , physics
Abstract Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS) of proteins was performed on a range of polytetrafluoroethylene (PTFE) surfaces. Sinapinic acid and α ‐cyano‐4‐hydroxycinnamic acid matrices were compared and the order of application varied to identify the best combination for each surface. It is demonstrated that the use of a PTFE surface improves the intensity of signals obtained for higher molecular weight proteins. Copyright © 2003 John Wiley & Sons, Ltd.