Improved detection of higher molecular weight proteins by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry on polytetrafluoroethylene surfaces | Zendy

Botting Catherine H. | Zendy

Premium

Improved detection of higher molecular weight proteins by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry on polytetrafluoroethylene surfaces

Author(s) -

Botting Catherine H.

Publication year - 2003

Publication title -

rapid communications in mass spectrometry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.528

H-Index - 136

eISSN - 1097-0231

pISSN - 0951-4198

DOI - 10.1002/rcm.954

Subject(s) - chemistry , mass spectrometry , desorption , polytetrafluoroethylene , surface enhanced laser desorption/ionization , matrix assisted laser desorption/ionization , matrix (chemical analysis) , matrix assisted laser desorption electrospray ionization , ionization , analytical chemistry (journal) , chromatography , time of flight mass spectrometry , sample preparation in mass spectrometry , laser , protein mass spectrometry , atmospheric pressure laser ionization , electrospray ionization , organic chemistry , ion , photoionization , adsorption , optics , physics

Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS) of proteins was performed on a range of polytetrafluoroethylene (PTFE) surfaces. Sinapinic acid and α ‐cyano‐4‐hydroxycinnamic acid matrices were compared and the order of application varied to identify the best combination for each surface. It is demonstrated that the use of a PTFE surface improves the intensity of signals obtained for higher molecular weight proteins. Copyright © 2003 John Wiley & Sons, Ltd.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research