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Analysis of unknown compounds in azithromycin bulk samples with liquid chromatography coupled to ion trap mass spectrometry
Author(s) -
Debremaeker D.,
Visky D.,
Chepkwony H. K.,
Van Schepdael A.,
Roets E.,
Hoogmartens J.
Publication year - 2003
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.917
Subject(s) - chemistry , chromatography , mass spectrometry , ion trap , atmospheric pressure chemical ionization , analytical chemistry (journal) , liquid chromatography–mass spectrometry , chemical ionization , direct electron ionization liquid chromatography–mass spectrometry interface , ion , ionization , organic chemistry
A selective reversed‐phase liquid chromatography/mass spectrometry (LC/MS n ) method is described for the identification of azithromycin impurities and related substances in commercial azithromycin samples. Mass spectral data are acquired on an LCQ ion trap mass spectrometer equipped with an atmospheric pressure chemical ionization interface operated in positive ion mode. The LCQ provides on‐line LC/MS n capability, making it ideally suited for identification purposes. In comparison with UV detection, this hyphenated technique provides as its main advantage efficient identification of novel substances without time‐consuming isolation and purification procedures. Using this technique, six novel related substances detected in commercial azithromycin samples have been studied. Copyright © 2003 John Wiley & Sons, Ltd.