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Determination of Tripamide in human urine by high‐performance liquid chromatography and high‐performance liquid chromatography/electrospray ionization tandem mass spectrometry
Author(s) -
Kim Yunje,
Hwang Joungboon,
Kim Myungsoo,
Lee Won
Publication year - 2003
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.913
Subject(s) - chemistry , chromatography , high performance liquid chromatography , mass spectrometry , electrospray ionization , tandem mass spectrometry , liquid chromatography–mass spectrometry , chromatography detector , direct electron ionization liquid chromatography–mass spectrometry interface , urine , electrospray , chemical ionization , ionization , organic chemistry , biochemistry , ion
Tripamide is a drug widely used in clinical practice for the treatment of hypertension and edema. This work evaluated a screening method for Tripamide and its urinary metabolites in human urine, using high‐performance liquid chromatography diode‐array detection (HPLC/DAD). Identification of these metabolites was investigated by high‐performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC/ESI‐MS/MS) after dosing with 15 mg Tripamide. Acid hydrolysis showed that Tripamide is conjugated in the body. Two suspected metabolites were detected by HPLC/DAD. HPLC/ESI‐MS/MS analysis suggested that these metabolites were probably hydroxylated together with loss of the ‐NH 2 group and dehydrogenation. These results will be useful in confirmation methods for Tripamide in doping control. Copyright © 2003 John Wiley & Sons, Ltd.
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