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Monitoring the three‐dimensional distribution of endogenous species in the lungs by matrix‐assisted laser desorption/ionization mass spectrometry imaging
Author(s) -
Flinders Bryn,
Morrell Josie,
Marshall Peter S.,
Ranshaw Lisa E.,
Heeren Ron M.A.,
Clench Malcolm R.
Publication year - 2020
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.8957
Subject(s) - mass spectrometry imaging , chemistry , analyte , mass spectrometry , parenchyma , matrix assisted laser desorption/ionization , lung , desorption , pathology , biomedical engineering , chromatography , organic chemistry , adsorption , medicine , linguistics , philosophy
Rationale Matrix‐assisted laser desorption/ionization mass spectrometry imaging (MALDI‐MSI) is routinely employed to monitor the distribution of compounds in tissue sections and generate two‐dimensional (2D) images. Whilst informative the images do not represent the distribution of the analyte of interest through the entire organ. The generation of 3D images is an exciting field that can provide a deeper view of the analyte of interest throughout an entire organ. Methods Serial sections of mouse and rat lung tissue were obtained at 120 μm depth intervals and imaged individually. Homogenate registration markers were incorporated in order to aid the final 3D image construction. Using freely available software packages, the images were stacked together to generate a 3D image that showed the distribution of endogenous species throughout the lungs. Results Preliminary tests were performed on 16 serial tissue sections of mouse lungs. A 3D model showing the distribution of phosphocholine at m/z 184.09 was constructed, which defined the external structure of the lungs and trachea. Later, a second experiment was performed using 24 serial tissue sections of the left lung of a rat. Two molecular markers, identified as [PC (32:1) + K] + at m/z 770.51 and [PC (36:4) + K] + at m/z 820.52, were used to generate 3D models of the parenchyma and airways, respectively. Conclusions A straightforward method to generate 3D MALDI‐MS images of selected molecules in lung tissue has been presented. Using freely available imaging software, the 3D distributions of molecules related to different anatomical features were determined.