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Simultaneous determination of succinylcholine and its metabolite in animal‐derived foods by solid‐phase extraction combined with ultra‐performance liquid chromatography/mass spectrometry
Author(s) -
Liu Sijie,
Zhang Bo,
Shi Mao,
Wang Hui,
Fu Yao
Publication year - 2020
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.8888
Subject(s) - chemistry , chromatography , metabolite , solid phase extraction , formic acid , electrospray ionization , extraction (chemistry) , ammonium acetate , detection limit , mass spectrometry , selected reaction monitoring , high performance liquid chromatography , tandem mass spectrometry , biochemistry
Rationale Succinylcholine has been increasingly used in the theft of animals. Because of the presence of residual levels of succinylcholine in poisoned animals, it is harmful for people to eat foods derived from these animals. Therefore, a method should be immediately established to determine succinylcholine and its metabolite in animal‐derived foods. Methods A fast, highly sensitive method, combining solid‐phase extraction (SPE) with ultra‐performance liquid chromatography/electrospray ionization tandem mass spectrometry (UPLC/ESI‐MS/MS), was developed for the determination of succinylcholine and its metabolite in animal‐derived foods. The sample was initially extracted with heptafluorobutyric acid and then further cleaned up using an SPE cartridge. Succinylcholine and its metabolite were separated using acetonitrile: 0.1% formic acid in 5 mmol L −1 ammonium acetate as the mobile phase. Quantitative results were based on positive ion ESI multiple reaction monitoring mode. Results The results show good linearity over a wide range with correlation coefficients of determination of more than 0.998. Both the limits of detection of succinylcholine and succinylmonocholine are 0.2 μg kg −1 . The intra‐ and inter‐day accuracies of the method are in the range 91.4%–104.6%, and the intra‐ and inter‐day precisions are in the range 2.5%–6.6%. Conclusions This method can be used for the determination of succinylcholine as an illicit drug in animal‐derived foods. It was successfully applied to the identification and quantification of succinylcholine and succinylmonocholine in animal‐derived foods collected from a local farmers market in Jilin Province of China.