Premium
Quantitative analysis of clindamycin in human plasma by liquid chromatography/electrospray ionisation tandem mass spectrometry using d 1 ‐ N ‐ethylclindamycin as internal standard
Author(s) -
Rechberger Gerald N.,
Fauler Günter,
Windischhofer Werner,
Köfeler Harald,
Erwa Wolfgang,
Leis HansJörg
Publication year - 2002
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.887
Subject(s) - chemistry , chromatography , mass spectrometry , electrospray , electrospray ionization , extraction (chemistry) , tandem mass spectrometry , pharmacokinetics , quantitative analysis (chemistry) , liquid chromatography–mass spectrometry , sample preparation , analytical chemistry (journal) , medicine
A new method for the quantitative analysis of clindamycin in human plasma by liquid chromatography/electrospray ionisation tandem mass spectrometry (LC/ESI‐MS/MS) is presented. Recently published methods possess a disadvantage because of their use of internal standards with extraction and ionisation properties different from those of clindamycin. To avoid these problems, d 1 ‐ N ‐ethylclindamycin was synthesised for use as internal standard by N‐demethylation and subsequent d 1 ‐N‐ethylation. Plasma sample preparation was done by an easy and rapid liquid–liquid extraction using ethyl acetate. The method was validated in the expected concentration range for a pharmacokinetic study. Calibration graphs were linear within the range 0.05–3.2 μg/mL plasma. Intra‐day precision was between 0.90% (2.8 μg/mL) and 3.25% (0.05 μg/mL), inter‐day variability was found to be between 1.33% (0.7 μg/mL) and 2.60% (0.05 μg/mL). Inter‐day accuracy showed deviations between 0.4% (0.05 μg/mL) and −4.8% (0.2 μg/mL). The method is simple and robust, and has been applied to the batch analysis of clindamycin during a pharmacokinetic study. Copyright © 2002 John Wiley & Sons, Ltd.