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Development of ultrahigh‐performance liquid chromatography/mass spectrometry and ultrahigh‐performance supercritical fluid chromatography/mass spectrometry assays to determine the concentration of Bitrex™ and sodium saccharin in homemade facemask fit testing solutions
Author(s) -
Herniman Julie M.,
Langley G. John
Publication year - 2020
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.8848
Subject(s) - chemistry , chromatography , mass spectrometry , supercritical fluid chromatography , electrospray ionization , high performance liquid chromatography , ammonium acetate , elution , analytical chemistry (journal)
Rationale Fast and easily transferable chromatography/mass spectrometry assays were required to detect and quantify the amount of Bitrex™ and sodium saccharin in homemade facemask fit testing solutions. Methods Bitrex™ solutions were analysed using reversed‐phase ultrahigh‐performance liquid chromatography coupled with positive ion electrospray ionisation mass spectrometry (UHPLC/ESI‐MS). Separation was achieved using a mobile phase gradient with an Acquity BEH C18‐packed column. Sodium saccharin solutions were analysed using ultrahigh‐performance supercritical fluid chromatography coupled with negative ion electrospray ionisation (UHPSFC/ESI‐MS). Separation was achieved using isocratic elution with an Acquity UPC 2 Torus Diol packed column and a methanol (25 mM ammonium acetate) co‐solvent. Results The calibration curves obtained using the ratio of the active compound to an internal standard generated linear regression values (R 2 ) >0.99. Samples analysed prior to and after an autoclave sterilisation process and bottling gave repeatable measurements within 10% of the expected concentration. Conclusions The two assays afford a fast robust and quantitative analytical method for the detection of the active components used to test the efficacy of the homemade facemask testing solutions.