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Fragmentation study of salinomycin and monensin A antibiotics using electrospray quadrupole time‐of‐flight mass spectrometry
Author(s) -
Miao XiuSheng,
March Raymond E.,
Metcalfe Chris D.
Publication year - 2002
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.882
Subject(s) - monensin , chemistry , salinomycin , fragmentation (computing) , mass spectrum , ion , mass spectrometry , electrospray , electrospray ionization , adduct , analytical chemistry (journal) , stereochemistry , chromatography , antibiotics , organic chemistry , biochemistry , computer science , operating system
The fragmentation pathways of two selected ionophore antibiotics, salinomycin and monensin A, were studied using electrospray (ES) orthogonal acceleration quadrupole time‐of‐flight mass spectrometry in positive‐ion mode. The identity of fragment ions was determined by accurate‐mass measurements. In ES mass spectra, ion signals of relatively high intensity were observed for [M+Na] + and [M−H+2Na] + for each antibiotic. Each of the ion species [M+Na] + and [M−H+2Na] + for salinomycin and [M−H+2Na] + for monensin A were isolated in turn and subjected to fragmentation. In the fragmentation of [M+Na] + and [M−H+2Na] + from salinomycin, only CC single bond cleavage and dehydration were observed. Product ion mass spectra obtained from [M−H+2Na] + of monensin A showed that ether ring opening, CC single bond cleavage and dehydration fragmentations had occurred. Fragment ions containing two sodium atoms were observed in the product ion mass spectrum of [M−H+2Na] + from salinomycin, but not from monensin A. Both type A (containing the terminal carboxyl group) and type F (containing the terminal hydroxyl group) fragment ions were observed in the product ion mass spectra of sodium adduct ions of salinomycin and monensin A. Copyright © 2002 John Wiley & Sons, Ltd.