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Metal ion complexes in the structural analysis of phospholipids by electrospray ionization tandem mass spectrometry
Author(s) -
Ho YenPeng,
Huang PingChen,
Deng KuangHao
Publication year - 2002
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.880
Subject(s) - chemistry , electrospray ionization , mass spectrometry , tandem mass spectrometry , ion , electrospray , metal ions in aqueous solution , reagent , quadrupole ion trap , transition metal , ion trap , metal , dissociation (chemistry) , analytical chemistry (journal) , chromatography , organic chemistry , catalysis
Abstract The effects of metal cationization on collisionally activated dissociation (CAD) of phospholipids were investigated by electrospray ionization with quadrupole ion trap tandem mass spectrometry. The metal ions include Li + , Na + , K + , Sr 2+ , Ba 2+ , and the first transition series. CAD of the transition metal ion‐bound lipid complexes gave significant yields of product ions that identify the positions of the two fatty acyl substituents on the glycerophospholipid backbone. The cobalt(II) ion, which has a single naturally occurring isotope, was expected to be a better cationization reagent as it produces simpler mass spectra than other transition metal ions. CAD of the cobalt(II) ion complexes of glycerophosphoethanolamines, glycerophosphoglycerols and glycerophosphoserines yielded product ions that revealed information regarding both the lipid classes and the regiospecific positions of the two fatty acyl substituents. Copyright © 2002 John Wiley & Sons, Ltd.