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Metabolic profile of alkaloids in Rhizoma Coptidis in rat plasma, urine and feces after oral administration using ultra‐high‐performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry
Author(s) -
Liu Yi,
Zhang Ye,
Meng Qi,
Long Jianglan,
Wang Man,
Zhang Xu,
Yan Can,
Yan Dan
Publication year - 2020
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.8763
Subject(s) - chemistry , chromatography , glucuronidation , in vivo , sulfation , oral administration , mass spectrometry , urine , hydroxylation , demethylation , metabolic pathway , high performance liquid chromatography , pharmacology , metabolism , biochemistry , microsome , gene , dna methylation , biology , enzyme , medicine , gene expression , microbiology and biotechnology
Rationale Rhizoma Coptidis (RC) has been used to treat diabetes, pertussis, bacillary dysentery, sore throat, eczema, and aphtha for thousands of years. Alkaloids are the major components in RC, and its curative effect is achieved by oral administration. However, information on its composition in vivo is weak. Methods In this study, ultra‐high‐performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry (UPLC/QTOF‐MS) was used to analyze the major active components and their metabolites in rat plasma, urine and feces after oral administration of RC extract. Results A total of 96 compounds including 8 prototype compounds and 88 metabolites were identified, and hydroxylation, reduction, demethylenation, demethylation, dehydrogenation, sulfation, glucuronidation and methylation were the major metabolic pathways. Conclusions This study analyzed metabolic processes of the major active components in RC in vivo , which provided important information for its active composition and in vivo mechanism research. Meanwhile, metabolic profile studies on representative compounds provided valuable reference materials to elucidate the full‐scale metabolites of RC.