Premium
Solid‐phase microextraction liquid chromatography/tandem mass spectrometry for the analysis of chlorophenols in environmental samples
Author(s) -
Sarrión M. N.,
Santos F. J.,
Moyano E.,
Galceran M. T.
Publication year - 2002
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.873
Subject(s) - chemistry , chromatography , mass spectrometry , atmospheric pressure chemical ionization , detection limit , solid phase microextraction , tandem mass spectrometry , selected reaction monitoring , chemical ionization , analytical chemistry (journal) , triple quadrupole mass spectrometer , fragmentation (computing) , gas chromatography–mass spectrometry , ionization , ion , organic chemistry , computer science , operating system
Liquid chromatography with atmospheric pressure chemical ionisation mass spectrometry (LC/APCI‐MS), using negative ion detection in a triple quadrupole instrument, was used for the determination of chlorophenols (CPs) in environmental samples. In‐source collision‐induced dissociation (CID) was compared with MS/MS fragmentation. In general, less fragmentation was observed in MS/MS as compared with in‐source CID, with the latter providing more intense fragment ions due to chemical ionisation. Under MS/MS conditions [M − H − HCl] − was the main fragment ion observed for all compounds except for pentachlorophenol, which showed no fragmentation. For multiple reaction monitoring (MRM) acquisition mode, the transition from [M − H] − to [M − H − HCl] − was selected, leading to detection limits down to 0.3 ng injected. Direct and headspace‐solid‐phase microextraction (HS‐SPME) were used as preconcentration procedures for the analysis of CPs in wood and in industrially contaminated soils. CPs were quantified by standard addition, which led to good reproducibility (RSD between 4 and 11%) in both SIM and MRM modes, and detection limits down to ng/g. The combination of MS/MS and in‐source CID allowed confirmation of the presence of CPs in environmental samples. Copyright © 2002 John Wiley & Sons, Ltd.