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Systematic identification of compounds in Macleaya microcarpa by high‐performance liquid chromatography/quadrupole time‐of‐flight tandem mass spectrometry combined with mass spectral fragmentation behavior of Macleaya alkaloids
Author(s) -
Lin Li,
Li XiaoYing,
Liu ShaSha,
Qing ZhiXing,
Liu XiuBin,
Zeng JianGuo,
Liu ZhaoYing
Publication year - 2020
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.8715
Subject(s) - sanguinarine , chemistry , quadrupole time of flight , tandem mass spectrometry , mass spectrometry , chromatography , selected reaction monitoring , high resolution , isoquinoline , alkaloid , stereochemistry , remote sensing , geology
Rationale Macleaya microcarpa (Maxim.) Fedde belongs to the genus Macleaya of the Papaveraceae family. Benzylisoquinoline alkaloids (BIAs) are considered the main bioactive constituents of M. microcarpa . Methods Using high‐performance liquid chromatography/quadrupole time‐of‐flight tandem mass spectrometry (HPLC/QTOFMS/MS) we identified BIAs in the aerial parts of M. microcarpa in the early flowering stage. Target profiling and identification of BIAs in the extracted samples from the fresh aerial parts of M. microcarpa were exclusively based on a personal, accurate, mass database of known compounds and the mass spectral fragmentation behavior of Macleaya alkaloids. Results A total of 97 alkaloids, comprising 7 benzyltetrahydroisoquinolines, 1aporphine, 9 tetraprotoberberines, 3 protoberberines, 2 N ‐methyltetrahydroprotoberberines, 4 protopines, 47 dihydrobenzophenanthridines, and 24 benzophenanthridines, were identified from the fresh aerial parts of M. microcarpa , and 77 of these were detected for the first time in M. microcarpa . In addition, some of the screened alkaloids were related to the biosynthetic pathways of sanguinarine and chelerythrine. Conclusions The integrated method is sensitive and reliable for screening and identifying trace or ultra‐trace isoquinoline alkaloids and has contributed to a better understanding of BIAs in the fresh aerial parts of M. microcarpa .