Premium
Development of sheet‐enhanced technique (Set) method for matrix‐assisted laser desorption/ionization imaging mass spectrometry
Author(s) -
Nakashima Yuko,
Eto Fumihiro,
Ishihara Kazuku,
Yamazaki Fumiyoshi,
Sato Shumpei,
Sakurai Takanobu,
Kahyo Tomoaki,
Setou Mitsutoshi
Publication year - 2020
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.8703
Subject(s) - chemistry , matrix (chemical analysis) , mass spectrometry , maldi imaging , mass spectrometry imaging , ionization , biomolecule , sample preparation , desorption , matrix assisted laser desorption electrospray ionization , matrix assisted laser desorption/ionization , analytical chemistry (journal) , chromatography , ion , biochemistry , organic chemistry , adsorption
Rationale The key to successful experiments in matrix‐assisted laser desorption/ionization imaging mass spectrometry (MALDI‐IMS) is to apply the matrix uniformly to the sample. With the development of automated equipment, uniform matrix application has made great progress while the sample preparation required to acquire a better image becomes complicated. Methods The approach is to apply the matrix uniformly to tape and adhere it to the tissue section. We call this the sheet‐enhanced technique (Set) method. Results The Set method promotes ionization of biomolecules as well as the spray method. This procedure does not require the preparation and application of a matrix solution for each experiment, dramatically reducing the time and effort of matrix deposition. Conclusions In the present study, we have developed the Set method as a new matrix application method. The method promotes ionization of biomolecules as well as the spray method for MALDI‐IMS.