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Improvement of m / z accuracy for linear matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometer
Author(s) -
Yu Jiajun,
Chudinov Alexey,
Li Lei,
Liu Jinzhao,
Gao Wei,
Huang Zhengxu,
Zhou Zhen,
Nikiforov Sergey,
Kozlovskiy Viatcheslav
Publication year - 2020
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.8701
Subject(s) - chemistry , mass spectrometry , time of flight mass spectrometry , analytical chemistry (journal) , ionization , mass spectrum , time of flight , matrix (chemical analysis) , resolution (logic) , ion , range (aeronautics) , spectral line , reflectron , computational physics , physics , chromatography , materials science , organic chemistry , astronomy , artificial intelligence , computer science , composite material
Rationale Linear matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS) is widely used in analytical and biomedical applications. The use of delayed extraction increases the resolution, but the roughness of the matrix crystals and the misalignment of the target plate in the order of a few micrometers cause a substantial spread in the ion TOF values and a decrease in mass accuracy. Methods The method of mass spectra correction based on the correlation of matrix fragment peaks in MALDI mass spectra was used. Experiments were performed using the MALDI‐TOF instrument CMI‐1600. SIMION 8.1 and MATLAB were used for ion motion simulations. Data analysis was done using the home‐built custom‐developed software and MATLAB. Results It was shown that the peak position drift in the MALDI‐TOF mass spectra depends linearly on the TOF in a wide mass range. While using the linear correction of the TOF scale, an increase in m / z accuracy of more than 10 times was achieved. The mass accuracy was limited by the resolution of the fast Analog‐to‐Digital Converter (ADC) used. Conclusions It is expected that the proposed method will significantly increase the dynamic range, since it becomes possible to sum up corrected individual mass spectra without a significant loss of resolution. Timescale adjusting can be used for both linear TOF instruments and reflector systems of various configurations.