Premium
Simple and sensitive method for the analysis of 14 antituberculosis drugs using liquid chromatography/tandem mass spectrometry in human plasma
Author(s) -
Lei Qian,
Zhao Yuan,
Wang Hao,
Zhou Jun,
Lv Xiaohui,
Dang Liyun,
Zhu Changsheng
Publication year - 2020
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.8667
Subject(s) - chemistry , chromatography , clofazimine , ethambutol , pyrazinamide , moxifloxacin , selected reaction monitoring , formic acid , tandem mass spectrometry , rifapentine , mass spectrometry , pharmacokinetics , gatifloxacin , liquid chromatography–mass spectrometry , therapeutic drug monitoring , levofloxacin , pharmacology , rifampicin , tuberculosis , mycobacterium tuberculosis , antibiotics , medicine , biochemistry , leprosy , dermatology , pathology , latent tuberculosis
Rationale Monitoring plasma concentration and adjusting doses of antituberculosis (TB) drugs are beneficial for improving responses to drug treatment and avoiding adverse drug reactions. A simple and sensitive liquid chromatography/tandem mass spectrometry method was developed to measure the plasma concentrations of 14 anti‐TB drugs: ethambutol, isoniazid, pyrazinamide, levofloxacin, gatifloxacin, moxifloxacin, prothionamide, linezolid, rifampin, rifapentine, rifabutin, cycloserine, p ‐aminosalicylic acid, and clofazimine. Methods Human plasma was precipitated by acetonitrile and was subsequently separated by an AQ‐C18 column with a gradient elution. Drug concentrations were determined using multiple reaction monitoring in positive ion electrospray ionization mode. According to pharmacokinetic data of patients, the peak concentration ranges and the timing of blood collection were determined. Results Intra‐ and interday precision was < 14.8%. Linearity, accuracy, extraction recovery, and matrix effect were acceptable for each drug. The stability of the method satisfied different storage conditions. Conclusions The method allowed the sensitive and reproducible determination of 14 frequently used anti‐TB drugs which has already been of benefit for some TB patients.