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Increasing clinical liquid chromatography/tandem mass spectrometry assay throughput using a full calibration curve generated by one injection from a single‐tube calibrator
Author(s) -
Cournoyer Jason J.,
Dey Subhakar
Publication year - 2020
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.8632
Subject(s) - analyte , chemistry , chromatography , calibration , calibration curve , mass spectrometry , isotopologue , tube (container) , vial , analytical chemistry (journal) , throughput , liquid chromatography–mass spectrometry , detection limit , computer science , materials science , statistics , telecommunications , organic chemistry , molecule , composite material , wireless , mathematics
Rationale Mass spectrometry (MS) generally delivers more accurate results than immunoassay (IA) for certain clinically relevant analytes, but IA is still the more prevalent methodology used by clinical laboratories because of barriers to MS adoption, such as lower throughput. Therefore, it is increasingly important to develop new strategies to increase LC/MS/MS throughput so that more accurate results can be delivered to patients and clinicians. Methods Throughput can be increased by reducing assay calibration time using a single‐tube calibrator, a mix of isotopologues of the target analyte at different concentrations in a biological matrix, rather than a set of traditional, multiple‐tube calibrators. One injection from a single‐tube calibrator can generate a full calibration curve such that each calibration point is from the multiple reaction monitoring (MRM) signal corresponding to a specific isotopologue. Results In this study, a single‐tube calibrator (five levels in one vial) and a set of multiple‐tube calibrators (seven levels in seven vials) were used to measure the concentration of testosterone in 42 serum samples originally value assigned by the Centers for Disease Control and Prevention (CDC) reference method. The bias between the CDC reference method and the single‐tube calibrator measurements and the multiple‐tube calibrators measurements was +1.1% and − 5.5%, respectively. These results were within the CDC Hormone Standardization (HoSt) program bias acceptance criteria of ±6.4%. Conclusions The results show that LC/MS/MS throughput can be increased using a single‐tube calibrator because it reduces assay calibration time while delivering equivalent results to those generated using traditional, multiple‐tube calibrators. The single‐tube calibrator may also save cost to laboratories through reductions in consumable consumption, technician labor time, and inventory management, as well as to manufacturers because fewer vials would need to be manufactured, tested, stored, and shipped.