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Analysing Sr isotopes in low‐Sr samples such as single insects with inductively coupled plasma tandem mass spectrometry using N 2 O as a reaction gas for in‐line Rb separation
Author(s) -
Murphy David Thomas,
Allen Charlotte M.,
Ghidan Osama,
Dickson Andrew,
Hu WanPing,
Briggs Ethan,
Holder Peter W.,
Armstrong Karen F.
Publication year - 2020
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.8604
Subject(s) - chemistry , nist , inductively coupled plasma mass spectrometry , isotope , analytical chemistry (journal) , strontium , mass spectrometry , isotopes of strontium , radiochemistry , environmental chemistry , chromatography , nuclear physics , physics , organic chemistry , natural language processing , computer science
Rationale Strontium isotopes are valuable markers of provenance in a range of disciplines. Limited amounts of Sr in low‐mass samples such as insects mean that conventional Sr isotope analysis precludes their use for geographic origins in many ecological studies or in applications such as biosecurity. Here we test the viability of using inductively coupled plasma tandem mass spectrometry (ICP‐MS/MS) with N 2 O as a reaction gas for accurately determining Sr isotopes in insects with Sr < 100 ng. Methods Strontium isotopes were determined in solution mode using ICP‐MS/MS with 0.14 L/min N 2 O as a reaction gas to convert Sr + into SrO + for in‐line separation of 87 Sr from 87 Rb. The Sr isotope reference standards NIST SRM 987, NIST SRM 1570a and NIST SRM 1547 were used to assess accuracy and reproducibility. Ten insect species collected from the wild as a proof‐of‐principle application were analysed for Sr concentration and Sr isotopes. Results Using ICP‐MS/MS we show for the first time that internal mass bias correction of 87 Sr 16 O/ 86 Sr 16 O based on 88 Sr 16 O/ 86 Sr 16 O works to give for NIST SRM 987 a 87 Sr/ 86 Sr ratio of 0.7101 ± 0.012 (RSD = 0.17%) and for NIST SRM 1570a a 87 Sr/ 86 Sr ratio of 0.7100 ± 0.009 (RSD = 0.12%), which are within error of the accepted values. The first 87 Sr/ 86 Sr ratio of NIST SRM 1547 is 0.7596 ± 0.0014. Strontium analyses were run on 0.8 mL of 0.25–0.5 ppb Sr, which equates to 2–4 ng of Sr. Strontium isotope analysis with a precision of >99.8% can be achieved with in‐line separation of 87 Sr from 87 Rb at least up to solutions with 25 ppb Rb. Conclusions A minimum of 5 mg of insect tissue is required for Sr isotope analysis. This new ICP‐MS/MS method enables Sr isotope analysis in single insects, allowing population‐scale studies to be feasible and making possible applications with time‐critical uses such as biosecurity.