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In situ analysis of oxytetracycline tablets based on matrix‐assisted laser desorption/ionization mass spectrometry imaging
Author(s) -
Xie Hanyi,
He Qichuan,
Zhao Yanfang,
Li Huijuan,
Zhao Mei,
Chen Xiangfeng,
Cai Zongwei,
Fang Kezhong,
Song Hexing
Publication year - 2020
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.8592
Subject(s) - chemistry , active ingredient , mass spectrometry , sample preparation , chromatography , mass spectrometry imaging , matrix (chemical analysis) , desorption , quantitative analysis (chemistry) , process engineering , pharmacology , medicine , engineering , organic chemistry , adsorption
Rationale A thorough understanding of the content and distribution of active ingredients in pharmaceuticals is essential for drug efficacy and safety. Technological advancements in mass spectrometry imaging present an opportunity for methodological innovation by providing qualification and quantification analysis, as well as spatial information, in the same assay, which has great potential for applications in the rapid analysis and quality control of drugs. Methods Matrix‐assisted laser desorption/ionization mass spectrometry imaging (MALDI‐MSI) was employed to directly analyze oxytetracycline tablets in order to map the distribution of the active constituent within the whole tablet. Quantitative analysis was capable of differentiating tablets containing various doses of the active pharmaceutical ingredient. Results To establish the methodology, detailed factors that influence matrix spraying and spatial resolution during sample preparation and the data acquisition process were optimized systematically. Quantitative analysis could differentiate the tablets containing various doses of the active compound. The proposed method was successfully applied to analyze real commercial tablets. Conclusions The developed method could successfully achieve the spatial location of oxytetracycline in actual tablet samples. These results could contribute to pharmaceutical tracing technology, especially the formulation process of tablets, which is helpful for monitoring the quality of pharmaceutical products and guaranteeing drug security.