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Floral volatiles identification and molecular differentiation of Osmanthus fragrans by neutral desorption extractive atmospheric pressure chemical ionization mass spectrometry
Author(s) -
Zhang Xiaoping,
Liu Jianchuan,
Wang Yanan,
Chingin Konstantin,
Hua Rong,
Zhu Liang,
Rahman Md. Matiur,
Frankevich Vladimir,
Chen Huanwen
Publication year - 2019
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.8554
Subject(s) - osmanthus fragrans , chemistry , mass spectrometry , linalool , atmospheric pressure chemical ionization , desorption , chemical ionization , chromatography , essential oil , environmental chemistry , organic chemistry , ionization , food science , ion , adsorption
Rationale Floral volatiles are commonly present only at trace amounts and can be degraded or lost during vapor collection, which is often challenging from the analytical standpoint. Osmanthus fragrans Lour. is a widely cultivated plant known for the highly distinct fragrance of its flowers. The identification of specific volatile organic compounds (VOCs) and molecular differentiation of O. fragrans without any chemical pretreatment and VOC collection are important. Methods Twenty‐eight VOCs released by the flowers from ten different cultivars of O. fragrans were identified using neutral desorption extractive atmospheric pressure chemical ionization mass spectrometry (ND‐EAPCI‐MS) without any chemical pretreatment or VOC collection. Chemical identification was performed by high‐resolution MS n analysis and whenever possible was confirmed by the analysis of standards. Results According to our literature search, nine of the identified VOCs, 3‐buten‐2‐one, cyclohexadiene, 2‐methylfuran, 3‐allylcyclohexene, cuminyl alcohol, hotrienol oxide, epoxy‐linalool oxide, N ‐(2‐hydroxyethyl) octanamide, and 3‐hydroxy‐dihydro‐β‐ionone, have not been reported in O. fragrans in earlier studies. Confident differentiation between ten different cultivars of O. fragrans was achieved by the principal component analysis of the mass spectrometric results. Conclusions The results of our ND‐EAPCI‐MS analysis substantially increase our knowledge about the chemistry of the O. fragrans floral fragrance and demonstrate the power of this technique for direct molecular profiling for plant recognition or in biotechnological applications.