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Electron capture dissociation of weakly bound polypeptide polycationic complexes
Author(s) -
Haselmann Kim F.,
Jørgensen Thomas J. D.,
Budnik Bogdan A.,
Jensen Frank,
Zubarev Roman A.
Publication year - 2002
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.853
Subject(s) - chemistry , dissociation (chemistry) , electron capture dissociation , photochemistry , computational chemistry , organic chemistry , mass spectrometry , chromatography , tandem mass spectrometry
We have previously reported that, in electron capture dissociation (ECD), rupture of strong intra molecular bonds in weakly bound supra molecular aggregates can proceed without dissociation of weak inter molecular bonds. This is now illustrated on a series of non‐specific peptide‐peptide dimers as well as specific complexes of modified glycopeptide antibiotics with their target peptide. The weak nature of bonding is substantiated by blackbody infrared dissociation, low‐energy collisional excitation and force‐field simulations. The results are consistent with a non‐ergodic ECD cleavage mechanism. Copyright © 2002 John Wiley & Sons, Ltd.