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Quantitative analysis using gas chromatography/combustion/isotope ratio mass spectrometry and standard addition of intrinsically labelled standards (SAIL) –application to isoflavones in foods
Author(s) -
Bluck Leslie J. C.,
Jones Kerry S.,
Thomas Jane,
Liggins Jason,
Harding Marilyn,
Bingham Sheila A.,
Coward W. Andrew
Publication year - 2002
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.850
Subject(s) - chemistry , daidzein , isotope ratio mass spectrometry , mass spectrometry , chromatography , isotope analysis , gas chromatography , extraction (chemistry) , combustion , genistein , isoflavones , gas chromatography–mass spectrometry , isotopes of carbon , environmental chemistry , organic chemistry , total organic carbon , medicine , ecology , biochemistry , biology
We have investigated a novel application of gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) for the quantitative analysis of the isoflavones in food matrices. Previous methods have been hampered by the lack of analytical standards to introduce early enough in the extraction procedure to allow compensations for losses at all stages of the procedure. In this work we have produced standard materials that can be added at the initial extraction, by intrinsically labelling soya plants by growing them in an atmosphere enriched in the stable isotope of carbon in CO 2 . On analysis these plants were shown to contain phytoestrogens at a high (up to 20%) level of enrichment. The dried plant material has been used to estimate the isoflavone concentrations of a set of spiked flours. For daidzein the methodology was shown to produce results comparable to those achieved by GC/MS techniques. The method was less successful for genistein, possibly due to the greater fragility of this compound under the conditions required for the analysis. Copyright © 2002 John Wiley & Sons, Ltd.