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Determination of melanotan‐II in rat plasma by liquid chromatography/tandem mass spectrometry: determination of pharmacokinetic parameters in rat following intravenous administration
Author(s) -
Mock S.,
Shen X.,
Tamvakopoulos C.
Publication year - 2002
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.847
Subject(s) - chemistry , chromatography , pharmacokinetics , analyte , solid phase extraction , tandem mass spectrometry , extraction (chemistry) , mass spectrometry , liquid chromatography–mass spectrometry , detection limit , selected reaction monitoring , pharmacology , medicine
A method is described for the determination of melanotan‐II (MT‐II), a synthetic cyclic heptapeptide analog of α‐melanocyte‐stimulating hormone (α‐MSH), in rat plasma by liquid chromatography/tandem mass spectrometry (LC/MS/MS). The analyte is recovered from plasma by solid‐phase extraction (SPE) and subsequently analyzed by LC/MS/MS. A SPE procedure using OASIS 96‐well plates is used for extraction of MT‐II from rat plasma. Using the described approach a limit of quantitation of 5 ng/mL was achieved in rat plasma. This level of sensitivity allowed the determination of pharmacokinetic parameters, following intravenous administration of MT‐II in rat. Copyright © 2002 John Wiley & Sons, Ltd.