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Tracking alterations of alkyl side chains of N 1 species in heavy crude oil after anaerobic biodegradation with negative‐ion electrospray ionization coupled with high‐field Fourier transform ion cyclotron resonance mass spectrometry
Author(s) -
Su Sanbao,
Dong Hao,
Yu Gaoming,
Hou Dujie,
Shi Quan,
Banat Ibrahim M.,
Wang Zhengliang,
Gu Yong'an,
Zhang Fan,
She Yuehui
Publication year - 2019
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.8422
Subject(s) - chemistry , fourier transform ion cyclotron resonance , electrospray ionization , biodegradation , naphthenic acid , mass spectrometry , nitrate , alkyl , ionization , ion , environmental chemistry , chromatography , organic chemistry , corrosion
Rationale Heteroatomic compounds are relatively abundant and believed to be bio‐resistant in heavy crude oils. However, few studies have focused on the biodegradation of these heteroatomic compounds. Methods Heteroatoms, especially N 1 species, in a blank crude oil and in three treated oils co‐incubated with anaerobic sulfate‐reducing bacteria, nitrate‐reducing bacteria and fermentative consortia cultures were detected using negative‐ion electrospray ionization coupled with high‐field Fourier transform ion cyclotron resonance mass spectrometry. Results The relative abundance of N 1 species in the three treated oils decreased, while the relative abundance of O 2 species increased. Remarkably, the relative abundances of N 1 species with low carbon number increased and those with higher carbon number decreased. Conclusions These results revealed that the anaerobic biodegradations of heavy crude oil occurred. With direct evidences, the degradations of alkyl side chains of N 1 species by the anaerobic microbes could be deduced.